From San Quirico d'Orcia, a very challenging stage of 32.7 km begins (which starts in the evening), with climbs and descents along relatively barren hills. You reach the small fortified village of Vignoni, with its view of the Val d'Orcia, and then descend to Bagno Vignoni, which is famous for its monumental thermal bath.
A long stretch up and down in the valleys of the rivers Orcia and Paglia brings us to the ancient hospital at Le Briccole. From here a tough climb up to the finishing line begins, ending at Radicofani, some 790 metres above sea level.
From Radicofani, there is another challenging stage of 31.8 km. The journey begins with an 8 km descent along the ridge, with views of the valleys that stretch toward the Lazio. Around you as far as the eye can see are hills and Monte Amiata, behind the Rocca di Radicofani. Once in Ponte a Rigo, the course runs along for several kilometres before entering Lazio, and then proceeds along a dirt road with views of the River Paglia valley, ending at Proceno.
The course descends to Ponte Gregoriano, before undertaking a final steep climb to the Ripa gate, entrance to the City of Acquapendente (The Jerusalem Of Europe), and the start of the Ultramarathon in Piazza del Comune.