A stage of 32 km begins in Siena. A challenging course along the roads of the Val d'Arbia, with panoramic views characteristic of the Siennese countryside, leading to Grancia di Cuna, an old fortified farmhouse owned by the hospital at Santa Maria della Scala.
Continuing along the foothills of the Crete Senesi, you get to Monteroni and finally reach Quinciano. A short distance away is the fortified village of Lucignano d'Arbia, with the Romanesque church of San Giovanni Battista.
After a stretch along the railway line, we reach Ponte d'Arbia and subsequently Buonconvento, the finishing line of the alternate route.