From Radicofani, there is another challenging stage of 31.8 km. The journey begins with an 8 km descent along the ridge, with views of the valleys that stretch toward the Lazio.
Around you as far as the eye can see are hills and Monte Amiata, behind the Rocca di Radicofani. Once in Ponte a Rigo, the course runs along for several kilometres before entering Lazio, and then proceeds along a dirt road with views of the River Paglia valley, ending at Proceno.
The course descends to Ponte Gregoriano, before undertaking a final steep climb to the Ripa gate, entrance to the City of Acquapendente (The Jerusalem Of Europe), and the start of the Ultramarathon in Piazza del Comune.